{"id":11651,"date":"2003-09-01T06:51:22","date_gmt":"2003-09-01T06:51:22","guid":{"rendered":"http:\/\/i-base.info\/htb\/?p=11651"},"modified":"2014-05-27T11:34:49","modified_gmt":"2014-05-27T11:34:49","slug":"more-mitochondrial-dna-depletion","status":"publish","type":"post","link":"https:\/\/i-base.info\/htb\/11651","title":{"rendered":"More mitochondrial DNA depletion"},"content":{"rendered":"

David Nolan presented updated results from the Western Australian HIV Cohort showing that adipocyte depletion and mitochondrial toxicity are prominent in subcutaneous fat samples from 46 RTI-treated patients, compared to 24 HIV-positive treatment na\u00efve patients and seven HIV-negative controls. [10]<\/strong><\/p>\n

Median mtDNA copies\/cell was 1,288 in treatment na\u00efve patients, and reduced by 81% to 240 copies\/cell in 28 patients using d4T and by 41% to 726 copies\/cell in 29 patients using AZT. Sequential biopsy samples showed significant changes in mtDNA within 2-12 months of initiated nucleoside therapy.<\/p>\n

These changes correlated with adipocyte toxicity \u2013 reduced size and disorganised tissue architecture and marked macrophage infiltration.<\/p>\n

Slides showed the differences between the \u2018plump normal fatty cells\u2019 seen in both HIV-negative and HIV-positive untreated individuals. Lipoatrophy is not a symptom of redistribution of fat \u2013 but one on specific fat cell damage in peripheral parts of the body.<\/p>\n

Lopez and colleagues reported that HIV antiretroviral na\u00efve patients had 68% mtDNA content with respect to HIV-negative control group, and that HIV itself could be a factor in mtDNA depletion [11] and Tebas and colleagues showed that mtDNA levels doubled on starting treatment, but fell again in patients when using d4T\/ddI. [12]<\/p>\n

Research on expression of SREBP-1 and apoB, showed increases in the synthesis and secretion of VLDL, and was suggested as an explanation for increases seen in levels of triglycerides and cholesterol. [13]<\/p>\n

<\/em>This article is part of a longer report from the\u00a05th International Workshop on Adverse Drug Reactions and Lipodystrophy,\u00a08-11 July 2003, Paris.\u00a0Part three of this report<\/a>.<\/em><\/p>\n

References:<\/p>\n

Unless otherwise stated, all abstracts refer to the programme and abstracts from the 5th International Workshop on Adverse Drug Reactions and Lipodystrophy in HIV, 8-11 July 2003, Paris and are published in Antiviral Therapy Volume 8 issue 4.<\/p>\n

    \n
  1. Nolan D, Hammond E, McKinnon E et al – Subcutaneous fat tissue mitochondrial DNA depletion and adipose toxicity are strongly associated with nucleoside reverse transcriptase inhibitor (NRTI) therapy in HIV-infected patients. Abstract 18.<\/span><\/li>\n
  2. Lopez S, Miro O, Martinez E et al \u2013 Does HIV infection itself have any effect on mitochondrial DNA content? Abstract 54.<\/span><\/li>\n
  3. Tebas P, Mondy K, De Ronde A et al \u2013 Effects of different antiretroviral treatments on mitochondrial DNA levels in PBMCs of HIV-infected patients. Abstract 60.<\/span><\/li>\n
  4. Shahmanesh M, Das S, Stolinski M et al – Effects of HIV infection, antiviral treatment and body fat changes on VLDL-apolipoprotein-B metabolism. Abstract 3.<\/span><\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

    David Nolan presented updated results from the Western Australian HIV Cohort showing that adipocyte depletion and mitochondrial toxicity are prominent in subcutaneous fat samples from 46 RTI-treated patients, compared to 24 HIV-positive treatment na\u00efve patients and seven HIV-negative controls. [10] …<\/p>\n","protected":false},"author":4,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4,27],"tags":[126],"class_list":["post-11651","post","type-post","status-publish","format-standard","hentry","category-conference-reports","category-weight-diabetes-metabolic-complications","tag-lipo-5th-2003"],"_links":{"self":[{"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/posts\/11651","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/comments?post=11651"}],"version-history":[{"count":0,"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/posts\/11651\/revisions"}],"wp:attachment":[{"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/media?parent=11651"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/categories?post=11651"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/tags?post=11651"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}