{"id":19803,"date":"2012-08-01T12:15:05","date_gmt":"2012-08-01T12:15:05","guid":{"rendered":"http:\/\/i-base.info\/htb\/?p=19803"},"modified":"2012-08-16T16:29:18","modified_gmt":"2012-08-16T16:29:18","slug":"defective-viral-reservoir-populations-is-common-in-patients-on-long-term-suppressed-art","status":"publish","type":"post","link":"https:\/\/i-base.info\/htb\/19803","title":{"rendered":"Defective viral reservoir populations is common in patients on long-term suppressed ART"},"content":{"rendered":"<p><strong>Simon Collins, HIV i-Base<\/strong><\/p>\n<p><strong>Fourati and colleagues from Paris analysed PRBC and rectal tissue samples from five patients maintained on controlled ART (range: 7\u201313 years) with five treatment-naive patients, using the presence of in-frame stop codon mutations in RT as an indicator of replication defective virus.<\/strong><\/p>\n<p>They reported a high level of defective genomes (median 21%; range 15%-100%) in the treated patients with the percentage inversely linked to the calculated size of the viral reservoir measured by proviral HIV DNA (r2=0.24; p=0.033). No similar mutations were found in the naive patients. Most of the changes were related to APOBEC3-induced hypermutations.<\/p>\n<p>The researchers proposed that their finding might support an accumulation of virus that is unable to replicate on ART, reaching a common viral extinction and that future use of proviral HIV DNA in reservoir sites in the context of cure research, should additionally measure whether this is replication competent.<\/p>\n<p>Reference:<\/p>\n<p>Fourati S et al. HIV-1 genome is often defective in PBMCs and rectal tissues after long-term HAART as a result of APOBEC3 editing and correlates with the size of reservoirs. 20th Intl Drug Resistance Workshop, 5\u20139 June 2012, Sitges. Abstract 33. Antiviral Therapy 2012: 17 Suppl 1:A41.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Simon Collins, HIV i-Base Fourati and colleagues from Paris analysed PRBC and rectal tissue samples from five patients maintained on controlled ART (range: 7\u201313 years) with five treatment-naive patients, using the presence of in-frame stop codon mutations in RT as &hellip;<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4,36],"tags":[158],"class_list":["post-19803","post","type-post","status-publish","format-standard","hentry","category-conference-reports","category-drug-resistance","tag-idrw-21st-2012"],"_links":{"self":[{"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/posts\/19803","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/comments?post=19803"}],"version-history":[{"count":0,"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/posts\/19803\/revisions"}],"wp:attachment":[{"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/media?parent=19803"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/categories?post=19803"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/tags?post=19803"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}