{"id":4702,"date":"2001-07-30T19:59:40","date_gmt":"2001-07-30T18:59:40","guid":{"rendered":"http:\/\/moomango.co.uk\/htb\/?p=4702"},"modified":"2014-06-11T17:20:39","modified_gmt":"2014-06-11T17:20:39","slug":"role-of-y318f-mutation-in-nnrti-resistance","status":"publish","type":"post","link":"https:\/\/i-base.info\/htb\/4702","title":{"rendered":"Role of Y318F mutation in NNRTI resistance"},"content":{"rendered":"<p><strong>Simon Collins and Polly Clayden, HIV i-Base<\/strong><\/p>\n<p><strong>Further information was provided by Kemp and colleagues on the role of the significance of the Y318F RT mutation in NNRTI resistance.<\/strong> [1]<\/p>\n<p>A search of genotype and phenotype results from the Virco database investigated this association and site-directed mutant viruses harbouring single or multiple NNRTI-associated mutations were constructed to determine phenotypic susceptibility.<\/p>\n<p>The presence of 318F predicted a &gt;10-fold increase in resistance, irrespective of other mutations in 83% isolates to efavirenz, 95% of isolates to nevirapine and 99% of isolates to delavirdine. The prevalence of 318 within the database was found to be 5% amongst those with K103N , 2% with Y181C and 15% with the delavirdine associated P236L mutation.<\/p>\n<p>318 alone in site-directed mutant virus only produced a &lt;3 fold increase in resistance to nevirapine or efavirenz (and 41-fold increase to delavirdine). In combination with either 103N, 181C or both this produced increases in resistance to efavirenz of 15-42-fold, 1.2-3.3-fold and 23-84-fold respectively.<\/p>\n<p>It may therefore be important to include this outer region of RT in genotypic assays when assessing NNRTI resistance.<\/p>\n<p>Reference:<\/p>\n<ol>\n<li>Kemp, SD et al &#8211; A mutation in HIV-1 reverse transcriptase at codon 318 (Y-F) confers high level NNRTI resistance in clinical samples. Antiviral Therapy 2001; 6 (Supplement1):36. Abstract 44.<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"<p>Simon Collins and Polly Clayden, HIV i-Base Further information was provided by Kemp and colleagues on the role of the significance of the Y318F RT mutation in NNRTI resistance. [1] A search of genotype and phenotype results from the Virco &hellip;<\/p>\n","protected":false},"author":4,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4,36],"tags":[112],"class_list":["post-4702","post","type-post","status-publish","format-standard","hentry","category-conference-reports","category-drug-resistance","tag-idrw-15th-2006"],"_links":{"self":[{"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/posts\/4702","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/comments?post=4702"}],"version-history":[{"count":0,"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/posts\/4702\/revisions"}],"wp:attachment":[{"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/media?parent=4702"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/categories?post=4702"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/tags?post=4702"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}