{"id":6574,"date":"2002-07-11T18:20:42","date_gmt":"2002-07-11T17:20:42","guid":{"rendered":"http:\/\/moomango.co.uk\/htb\/?p=6574"},"modified":"2014-06-05T11:17:08","modified_gmt":"2014-06-05T11:17:08","slug":"cd8-t-cells-from-hiv-exposed-but-uninfected-individuals-suppress-hiv-activity","status":"publish","type":"post","link":"https:\/\/i-base.info\/htb\/6574","title":{"rendered":"CD8+ T cells from HIV-exposed but uninfected individuals suppress HIV activity"},"content":{"rendered":"<p><strong>Graham McKerrow HIV i-Base<\/strong><\/p>\n<p><strong>CD8 T cells from individuals who remain uninfected despite being repeatedly exposed to HIV-1 (known as exposed-uninfected or EU) exert strong HIV-suppressive activity, according to researchers at the San Raffaele Scientific Institute in Milan.<\/strong><\/p>\n<p>Dr Lucinda Furci and colleagues studied the CD8 T lymphocytes from a well-characterised cohort of 16 HIV-1-discordant monogamous heterosexual couples. The lymphocytes were tested for their suppressive activity against HIV-1 strains displaying different coreceptor usage (one using coreceptor R5, one using X4, and one using both X4R5).<\/p>\n<p>To evaluate the in vivo functional competence of CD8 T cells, no ex vivo activatory stimuli were used prior to cocultivation with infected CD4+ T cells. In some experiments a semi-permeable membrane was used to separate CD4+ and CD8+ T cells.<\/p>\n<p>The researchers report in the journal AIDS of 3 May that unstimulated CD8 T cells from all but one of the EU individuals analysed effectively inhibited the growth of all HIV-1 strains, regardless of their coreceptor usage, with a mean potency similar to that of asymptomatic HIV-infected patients.<\/p>\n<p>\u201cThe HIV-inhibitory activity persisted for a long time after ceasing high risk-sexual behaviour, although a moderate decline was observed starting four years after the last risk episode.<\/p>\n<p>\u201cTranswell culture experiments showed that soluble factors are involved in CD8-mediated viral suppression, although the activity was higher when cell-to-cell contact was allowed,\u201d Dr Furci and colleagues write.<\/p>\n<p>They conclude: \u201cThese data demonstrate that CD8 T cells from EU individuals exert a strong, broad-spectrum HIV-suppressive activity, suggesting a role of non-cytotoxic antiviral mechanisms in resistance to HIV-1 infection.<\/p>\n<p>Reference:<\/p>\n<p>Furci L, Lopalco L, Loverro P et al. Non-cytotoxic inhibition of HIV-1 infection by unstimulated CD8+ T lymphocytes from HIV-exposed-uninfected individuals. AIDS 2002 May 3;16(7):1003-8<br \/>\n<a href=\"http:\/\/www.ncbi.nlm.nih.gov:80\/entrez\/query.fcgi?cmd=Retrieve&amp;db=PubMed&amp;list_uids=11953466\">http:\/\/www.ncbi.nlm.nih.gov:80\/entrez\/query.fcgi?cmd=Retrieve&amp;db=PubMed&amp;list_uids=11953466<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Graham McKerrow HIV i-Base CD8 T cells from individuals who remain uninfected despite being repeatedly exposed to HIV-1 (known as exposed-uninfected or EU) exert strong HIV-suppressive activity, according to researchers at the San Raffaele Scientific Institute in Milan. Dr Lucinda &hellip;<\/p>\n","protected":false},"author":4,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[15],"tags":[],"class_list":["post-6574","post","type-post","status-publish","format-standard","hentry","category-basic-science-and-immunology"],"_links":{"self":[{"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/posts\/6574","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/comments?post=6574"}],"version-history":[{"count":0,"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/posts\/6574\/revisions"}],"wp:attachment":[{"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/media?parent=6574"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/categories?post=6574"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/tags?post=6574"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}