{"id":7706,"date":"2005-01-31T08:39:50","date_gmt":"2005-01-31T08:39:50","guid":{"rendered":"http:\/\/moomango.co.uk\/htb\/?p=7706"},"modified":"2014-05-22T15:10:45","modified_gmt":"2014-05-22T15:10:45","slug":"weight-loss-is-associated-with-elevated-pbmc-proviral-dna-levels","status":"publish","type":"post","link":"https:\/\/i-base.info\/htb\/7706","title":{"rendered":"Weight loss is associated with elevated PBMC proviral DNA levels"},"content":{"rendered":"<p><strong>Graham McKerrow, HIV i-Base<\/strong><\/p>\n<p><strong>Weight loss in the HAART era may be driven by residual HIV infection in cells of the monocyte\/macrophage lineage, conclude Shikuma and colleagues from the University of Hawaii after a study involving 67 HIV-positive people, a subset of the Hawaii Aging with HIV longitudinal cohort study.<\/strong><\/p>\n<p>Catabolic cytokines such as TNF-alpha are often elevated in HIV-positive people who have received ARV therapy. Residual HIV infection in cells of the M\/M-omega lineage have been found in ARV-treated people who have undetectable plasma HIV-1 RNA.<\/p>\n<p>The researchers hypothesised that weight loss might be secondary to residual HIV infection in these cells with resultant increases in catabolic cytokine production and release.<\/p>\n<p>They analysed the patients\u2019 PBMC proviral DNA copies\/cell, select plasma cytokine levels and weight records over the last year of follow-up. Approximately 17% had weight loss &gt; 10 %, 15% had weight loss from &gt; 5% to 10%, 60% maintained stable weight (less than 5% gain or loss), and 6% gained weight.<\/p>\n<p>PBMC proviral DNA levels were higher in those with weight loss &gt; 5% compared to those with stable or increasing weight [median 8.9 vs 0.9 copies\/106 cells; p=0.006]. Proviral DNA levels remained higher in the 52% of subjects with plasma HIV RNA levels &lt; 50 copies\/mL [median 8.9 vs 0.5 copies\/106 cells, p=0.028]. Analysis of HIV DNA in subsets of PBMCs showed that the majority of proviral DNA copies in PBMCs were in activated (CD14+\/CD16+) macrophages.<\/p>\n<p>Reference:<\/p>\n<p>Shikuma C M, Valcour V, Ratto-Kim S et al. Loss of weight in the era of HAART is associated with elevated PBMC proviral DNA levels. 6th Lipodystrophy Workshop (6th IWADRLH), Washington, 2004. Abstract 57. Antiviral Therapy 2004; 9:L35.<br \/>\n<a href=\"http:\/\/www.aegis.org\/DisaplayConf\/?Abstract=87229\">http:\/\/www.aegis.org\/DisaplayConf\/?Abstract=87229<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Graham McKerrow, HIV i-Base Weight loss in the HAART era may be driven by residual HIV infection in cells of the monocyte\/macrophage lineage, conclude Shikuma and colleagues from the University of Hawaii after a study involving 67 HIV-positive people, a &hellip;<\/p>\n","protected":false},"author":4,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4,15],"tags":[127],"class_list":["post-7706","post","type-post","status-publish","format-standard","hentry","category-conference-reports","category-basic-science-and-immunology","tag-lipo-6th-2004"],"_links":{"self":[{"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/posts\/7706","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/comments?post=7706"}],"version-history":[{"count":0,"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/posts\/7706\/revisions"}],"wp:attachment":[{"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/media?parent=7706"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/categories?post=7706"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/i-base.info\/htb\/wp-json\/wp\/v2\/tags?post=7706"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}