Q and A

Question

What is the difference between the ELISA and ELFA test?

I am from India.

I had a possible exposure to HIV on 23rd Nov 2008 and tested for HIV on 25th Feb 2009 – 96 days after exposure. The HIV test result said HIV I and II (ELFA) – Non Reactive. A year later, on 2nd Feb 2010 I again got an HIV test done, the result reported was HIV I and II (ELFA) – Non Reactive.

What is the difference between ELFA and ELISA HIV tests?
Are my results conclusive?

Answer

Thank you for your question.

The Enzyme-Linked Fluourescent Assay (ELFA) is a slightly newer version of the Enzyme-Linked Immunosorbent Assay (ELISA) test. Both work in a similar way but the ELFA has a window period which is about 5 days shorter than the ELISA test.

Both are very accurate and will detect about 95% of infections at 28 days and more than 99.99% of infections at 90 days.

For more information on HIV testing please follow this link

4 comments

  1. Lisa Thorley

    Hi Romesh,

    Please see Q’s 1 and 8 here:

    http://i-base.info/qa/what-are-the-most-asked-questions

  2. Romesh

    ELFA test result is o.13. Is it hiv positive or negative.?

  3. Roy Trevelion

    We no longer answer questions about transmission and testing. This information service is for people who are HIV positive, and about HIV treatment.

    Most questions about transmission and testing have been answered here:
    http://i-base.info/qa/factsheets/hiv-transmission-and-testing

  4. pattrik

    I had a possible exposure to HIV on 2005 and on 2011I I got an HIV test done, the result reported were HIV p24ag(ELFA):negative and HIV 1&2 total ab(ELFA):negative,I wanted to see this result is reliable?
    .